The isolated muscle fibre as a model of disuse atrophy: characterization using PhAct, a method to quantify f-actin.

摘要: Research into muscle atrophy and hypertrophy is hampered by limitations of the available experimental models. Interpretation in vivo experiments confounded complexity environment while vitro models are subject to marked disparities between cultured myotubes mature myofibres living tissues. Here we develop a method (PhAct) based on ex maintenance isolated myofibre as model disuse atrophy, using standard microscopy equipment widely analysis software, measure f-actin content per nucleus over two weeks maintenance. We characterize 35% week terms early changes gene expression investigate effects loss mass modulatory agents, including Myostatin Follistatin. By tracing incorporation nucleotide analogue show that observed not associated with or replacement myonuclei. Such completely controlled investigation can be conducted single muscle. With this novel distinguish those features mechanisms intrinsic fibre from include activities other tissues systemic agents.