Cloning and characterization of VIGG, a novel virus-induced grapevine protein, correlated with fruit quality.

作者: Hironori Katoh , Shunji Suzuki , Toshiyuki Saitoh , Tsutomu Takayanagi

DOI: 10.1016/J.PLAPHY.2008.12.003

关键词:

摘要: We report here the identification and characterization of VIGG, a novel virus-induced grapevine protein. Analysis VIGG expression in demonstrated that was constitutively expressed leaves stems virus-infected grapevine, induced by virus A (GVA) infection, but not infection with other viruses. The profile supported finding virus-free meristem cultures prepared from grapevines did express VIGG. An experiment using GFP-VIGG fusion protein might be localized or around endoplasmic reticulum (ER). Treatment cells ER stress inducers resulted induction expression. Berries VIGG-expressing had higher organic acid phenolic contents than those control Interestingly, fruit composition simultaneously infected GVA B (GVB), which significantly different GVA-infected expressing suggesting effector alteration expression, infection. Taken together, suppress decrease content increase phenol berries. Further investigation biological function is expected to provide new information on quality grapevines.

参考文章(49)
Pyung Ok Lim, Hong Gil Nam, The molecular and genetic control of leaf senescence and longevity in Arabidopsis. Current Topics in Developmental Biology. ,vol. 67, pp. 49- 83 ,(2005) , 10.1016/S0070-2153(05)67002-0
P. Iocco, T. Franks, M.R. Thomas, Genetic transformation of major wine grape cultivars of Vitis vinifera L. Transgenic Research. ,vol. 10, pp. 105- 112 ,(2001) , 10.1023/A:1008989610340
P. Saldarelli, G. P. Martelli, A. Minafra, Vitivirus, a new genus of plant viruses. Archives of Virology. ,vol. 142, pp. 1929- 1932 ,(1997)
G. P. Martelli, Graft-transmissible diseases of grapevines: handbook for detection and diagnosis. Food and Agriculture Organization (FAO). ,(1993)
Shinya Kamauchi, Hiromi Nakatani, Chiharu Nakano, Reiko Urade, None, Gene expression in response to endoplasmic reticulum stress in Arabidopsis thaliana FEBS Journal. ,vol. 272, pp. 3461- 3476 ,(2005) , 10.1111/J.1742-4658.2005.04770.X
Toshiyuki Nagata, Yasuyuki Nemoto, Seiichiro Hasezawa, Tobacco BY-2 Cell Line as the “HeLa” Cell in the Cell Biology of Higher Plants International Review of Cytology-a Survey of Cell Biology. ,vol. 132, pp. 1- 30 ,(1992) , 10.1016/S0074-7696(08)62452-3
Roberta Piredda, R. garau, D. Boscia, Vanda A. Prota, U. Prota, On the possible relationship between Kober stem grooving and grapevine virus A. Vitis: Journal of Grapevine Research. ,vol. 33, pp. 161- 163 ,(1994)
Bruno Mezzetti, Tiziana Pandolfini, Oriano Navacchi, Lucia Landi, Genetic transformation of Vitis vinifera via organogenesis BMC Biotechnology. ,vol. 2, pp. 18- 18 ,(2002) , 10.1186/1472-6750-2-18
John L.R. Rubenstein, A. Elizabeth J. Brice, Roland D. Ciaranello, Dan Denney, Matthew H. Porteus, Ted B. Usdin, Subtractive hybridization system using single-stranded phagemids with directional inserts Nucleic Acids Research. ,vol. 18, pp. 4833- 4842 ,(1990) , 10.1093/NAR/18.16.4833
Elfriede M. Linsmaier, Folke Skoog, Organic Growth Factor Requirements of Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 18, pp. 100- 127 ,(1965) , 10.1111/J.1399-3054.1965.TB06874.X