BrainPhys neuronal medium optimized for imaging and optogenetics in vitro

摘要: Abstract The capabilities of imaging technologies, fluorescent sensors, and optogenetics tools for cell biology have improved exponentially in the last ten years. At same time, advances cellular reprogramming organoid engineering quickly expanded use human neuronal models vitro. Altogether this creates an increasing need tissue culture conditions better adapted to live-cell imaging. Here, we identified multiple caveats traditional media when used live functional assays on cultures (e.g., phototoxicity, suboptimal fluorescence signals, unphysiological activity). To overcome these issues, developed a new neuromedium, “BrainPhys™ Imaging”, which adjusted phototoxic compounds. medium is based formulation original BrainPhys medium, designed support activity neurons vitro1. We tested imaging-optimized cultured monolayers or organoids, rat primary neurons. Imaging enhanced signals reduced phototoxicity throughout entire light spectrum. Importantly, consistent with standard BrainPhys, showed that optimally supports electrical synaptic midbrain cortical culture. also benchmarked capacity calcium optogenetic control Altogether, our study shows improves quality wide range applications vitro while supporting viability functions.