Peripheral T-lymphocyte subpopulations in different clinical stages of chronic HBV infection correlate with HBV load
作者： Jing You , Lin Zhuang , Yi-Feng Zhang , Hong-Ying Chen , Hutcha Sriplung
DOI: 10.3748/WJG.15.3382
关键词:
摘要: AIM: To characterize the peripheral T-cell subpopulation profiles and their correlation with hepatitis B virus (HBV) replication in different clinical stages of chronic HBV infection. METHODS: A total 422 patients infection were enrolled this study. The divided into three stages: immune-tolerant stage, immune active immune-inactive carrier stage. Composition subpopulations was determined by flow cytometry. markers detected enzyme-linked immunosorbent assay. Serum DNA load assessed quantitative real-time polymerase chain reaction. RESULTS: CD8+ T-cells significantly higher at stage than immune-active -inactive (36.87 ± 7.58 vs 34.37 9.07, 36.87 28.09 5.64, P < 0.001). blood contained more CD4+ 30.23 6.35, 9.07 30.92 7.40, 0.01), whereas normal controls less (28.09 5.64 36.85 6.06, 24.02 4.35 38.94 3.39, 0.01). ANOVA linear trend test showed that increased a high viral (39.41 7.36, 33.83 7.50, 31.81 5.95 26.89 5.71, 0.001), while low (37.45 6.14, 33.33 5.61, 31.58 6.99 27.56 5.49, Multiple regression analysis displayed log copies still maintained its highly significant coefficients for subpopulations, strongest predictors variations CD3+, cells CD4+/CD8+ ratio after adjustment age HBV-infection, maternal HBV-infection status, presence e antigen mutation. CONCLUSION: Differences can be found infection. impairment is associated load.
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