High pressure gases: role of dynamic intracellular pH in pasteurization.

摘要: Certain dense gases, including CO2 and N2O, are known to deactivate food pathogens safely. Complete deactivation requires disabling intracellular metabolic pathways by extracellular processing that causes membrane disruption, irreversible denaturation of proteins, or extraction cell contents. At present, neither the precise physical mechanisms nor their kinetics behind gas pasteurization known. The depend strongly on both organism environment may combine permeabilization, altering pH. Herein we elucidate inactivation with aid a novel approach for measuring pH (pHi) under high pressure. Using pH-sensitive GFP-variant S. cerevisiae as probe, demonstrate permeabilization non-acidic gas, contributes but at rate is relatively low compared CO2. not only permeabilizes also brings about rapid drop in pHi, leading greater deactivation. Mechanistic understanding vital develop safe effective technologies treatment. Knowledge pHi important other cellular processes, enzyme activity, gene transcription, protein synthesis. GFP technique has been demonstrated be versatile even Bioeng. 2011; 108:1211–1214. © 2010 Wiley Periodicals, Inc.