Production and Characterization of Bacterial Cellulose by Leifsonia sp. CBNU-EW3 Isolated from the Earthworm, Eisenia fetida

作者: Palanivel Velmurugan , Hyun Myung , Muthusamy Govarthanan , Young-Joo Yi , Sang-Ki Seo

DOI: 10.1007/S12257-014-0793-Y

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摘要: A total of five bacterial strains were isolated from earthworm, Eisenia fetida and examined for cellulose (BC) production in Hestrin-Schramm medium (HS). Among the tested, CBNU-EW3 exhibited excellent BC was identified as Leifsonia sp. by 16S rDNA sequence analysis. optimum at pH 5, 30°C, with glucose yeast extract carbon nitrogen sources, respectively, according to 15 day-long experiments. (XRD) analysis dried pellicle indicated that partially crystalline type I. Fourier transform infrared spectroscopy (FT-IR) showed obtained contained same functional groups typical BC. Field emission scanning electron microscopy (FESEM) images micro-fibril matrix consisted a flat surface large pore size aggregation.

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