Experimental Toxoplasma gondii and Eimeria tenella co-infection in chickens.

摘要: The widespread apicomplexan parasites Toxoplasma gondii (T. gondii) and Eimeria tenella (E. tenella) are important pathogens with high prevalence in poultry. aim of our study was the investigation mutual influences co-infected chickens, focusing on immune response course infection. Two separate trials were performed using total 96 1-day-old divided into four groups: group NC (negative control, uninfected), PC-T (oral or intramuscular infection T. oocysts (trial 1) tachyzoites 2), respectively), PC-E E. acervulina 2)), TE (co-infection). infections validated by different parameters, cytokine expression gut spleen investigated. gondii-specific antibodies detected earliest 4 days post (p.i.) immunoblot direct DNA detection possible 22.1% all tissue samples from infected chickens. spp. merogony seemed to be enhanced co-infection gondii, interestingly without marked differences oocyst excretion between mono-infected An increase messenger RNA (mRNA) Th1- (IFN-γ, IL-12, TNF-α) Th2-related cytokines (IL-10) mainly groups observed, however, statistically significant single Eimeria. In conclusion, most measurable could attributed To best knowledge, this is first report experiments