Acoustic tweezers for studying intracellular calcium signaling in SKBR-3 human breast cancer cells

摘要: Extracellular matrix proteins such as fibronectin (FNT) play crucial roles in cell proliferation, adhesion, and migration. For better understanding of these associated cellular activities, various microscopic manipulation tools have been used to study their intracellular signaling pathways. Recently, it has appeared that acoustic tweezers may possess similar capabilities the study. Therefore, we here demonstrate our newly developed with a high-frequency lithium niobate ultrasonic transducer potentials calcium by FNT-binding human breast cancer cells (SKBR-3). It is found elevations SKBR-3 cells, initially occurring on microbead-contacted spot then eventually spreading over entire cell, are elicited attaching an acoustically trapped FNT-coated microbead. Interestingly, they suppressed either extracellular elimination or phospholipase C (PLC) inhibition. Hence, this suggests serve alternative tool activities.