Characterization of a highly invasive and spontaneously metastatic human malignant melanoma cell line.

摘要: Although the incidence of, and deaths due to, malignant melanoma are rising at a rapid rate, few experimental models mimic highly metastatic properties associated with pathogenesis of human disease, making study disease difficult. Thus, new required to understand biology, especially its properties. Here we describe C8161, invasive spontaneously cell line, which grows progressively in subcutis athymic nude mice an average doubling time approximately 6 days. By tumor reaches diameter 1 cm, amelanotic metastases lymph nodes, skin, peritoneal wall, spleen lungs have formed. comparing C8161 variants from other well-characterized melanomas (A375 MeWo) differing traits, presumed be involved propensity were examined. showed 2- 14-fold higher ability invade reconstituted basement membrane barriers MICS correspondingly high type-IV collagenase mRNA levels collagenolytic activity, as compared lines. Likewise, differential adhesion immobilized RBM or HUVEC monolayers was observed, but did not correlate rank orders Recently, correlation between surface expression ICAM-1 secondary formation by has been described several laboratories. Basal on A375 MeWo compared, no potential noted. Proto-oncogene cells A375P A375M using Northern blot analysis. c-myc 6-fold greater than both variants; c-fos 3.4-fold less 1.7-fold A375M; c-jun 2.5-fold 2.1-fold A375M, respectively. Because is so malignant, amenable manipulation, behavior mimics clinical course melanoma, this line will prove valuable for studying progression.