A sensitive method for the assay of serum prolyl endopeptidase.
作者： F Goossens , G Vanhoof , S Scharpé , I De Meester
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摘要: A new fluorimetric assay for the determination of prolyl endopeptidase (EC 3.4.21.26) was developed. The synthetic substrate Z-glycyl-prolyl-4-methylcoumarinyl-7-amide (0.2 mmol/l; 5 microliters), K-phosphate buffer (100 mmol/l, pH 7.5; 100 and serum (10 microliters) are incubated 120 min at 37 degrees C. reaction is stopped with acetic acid (1.5 mol/l; 500 released 7-amino-4-methylcoumarin measured fluorimetrically. mean value catalytic activity concentration in healthy volunteers 0.455 (SD = 0.092) mumol per litre minute. proposed procedure sensitive, robust, economical.
参考文章(12)
TADASHI YOSHIMOTO, HIROSHI OYAMA, NOBUHIRO KORIYAMA, DAISUKE TSURU, Prolyl endopeptidase from bovine testis: purification, characterization and comparison with the enzymes from other tissues. Chemical & Pharmaceutical Bulletin. ,vol. 36, pp. 1456- 1462 ,(1988) , 10.1248/CPB.36.1456
Tadashi Yoshimoto, Kiyokazu Ogita, Roderich Walter, Masao Koida, Daisuke Tsuru, Post-proline cleaving enzyme. Synthesis of a new fluorogenic substrate and distribution of the endopeptidase in rat tissues and body fluids of man. Biochimica et Biophysica Acta. ,vol. 569, pp. 184- 192 ,(1979) , 10.1016/0005-2744(79)90053-6
Akihiko Moriyama, Makoto Nakanishi, Makoto Sasaki, Porcine muscle prolyl endopeptidase and its endogenous substrates. Journal of Biochemistry. ,vol. 104, pp. 112- 117 ,(1988) , 10.1093/OXFORDJOURNALS.JBCHEM.A122404
Roderich Walter, WilliamH. Simmons, Tadashi Yoshimoto, Proline specific endo- and exopeptidases. Molecular and Cellular Biochemistry. ,vol. 30, pp. 111- 127 ,(1980) , 10.1007/BF00227927
Padraig BROWNE, Gerard O'CUINN, An evaluation of the role of a pyroglutamyI peptidase, a post‐proline cleaving enzyme and a post‐proline dipeptidyI amino peptidase, each purified from the soluble fraction of guinea‐pig brain, in the degradation of thyroliberin in vitro FEBS Journal. ,vol. 137, pp. 75- 87 ,(1983) , 10.1111/J.1432-1033.1983.TB07797.X
Sherwin Wilk, Marian Orlowski, Inhibition of Rabbit Brain Prolyl Endopeptidase byN-Benzyloxycarbonyl-Prolyl-Prolinal, a Transition State Aldehyde Inhibitor Journal of Neurochemistry. ,vol. 41, pp. 69- 75 ,(1983) , 10.1111/J.1471-4159.1983.TB11815.X
Antonio C.M. Camargo, Hildeberto Caldo, Piers C. Emson, Degradation of neurotensin by rabbit brain endo-oligopeptidase A and endo-oligopeptidase B (proline-endopeptidase) Biochemical and Biophysical Research Communications. ,vol. 116, pp. 1151- 1159 ,(1983) , 10.1016/S0006-291X(83)80263-0
Patrick E. Ward, Hester H. Bausback, Charles E. Odya, Kinin and angiotensin metabolism by purified renal post-proline cleaving enzyme. Biochemical Pharmacology. ,vol. 36, pp. 3187- 3193 ,(1987) , 10.1016/0006-2952(87)90631-9
R. Walter, H. Shlank, J. D. Glass, I. L. Schwartz, T. D. Kerenyi, Leucylglycinamide Released from Oxytocin by Human Uterine Enzyme Science. ,vol. 173, pp. 827- 829 ,(1971) , 10.1126/SCIENCE.173.3999.827
Takeshi Kato, Masamichi Okada, Toshiharu Nagatsu, Distribution of post-proline cleaving enzyme in human brain and the peripheral tissues Molecular and Cellular Biochemistry. ,vol. 32, pp. 117- 121 ,(1980) , 10.1007/BF00227437