Non-invasive genotyping with a massively parallel sequencing panel for the detection of SNPs in HPA-axis genes

摘要: We designed a genotyping panel for the investigation of genetic underpinnings inter-individual differences in aggression and physiological stress response. The builds on single nucleotide polymorphisms (SNPs) genes involved three subsystems hypothalamic-pituitary-adrenal (HPA)-axis: catecholamine, serotonin corticoid metabolism. To promote pipeline use with wild animal populations, we used non-invasively collected faecal samples from population Assamese macaques (Macaca assamensis). targeted loci 46 previously reported SNPs 21 candidate coding elements HPA-axis and amplified sequenced them using next-generation Illumina sequencing technology. compared multiple bioinformatics pipelines variant calling effect prediction. Based this strategy application different quality thresholds, identified up to 159 types predicted functional effects among our natural study population. This provides massively parallel that will facilitate integrating large-scale SNP data into behavioural studies. Such multi-faceted approach understanding flexibility constraints behaviour hormone physiology.