The interaction of carbamylated low-density lipoprotein with cultured cells. Studies with human fibroblasts, rat peritoneal macrophages and human monocyte-derived macrophages.

摘要: Abstract We determined the effects of various degrees chemical modification low-density lipoprotein (LDL) on its interaction with receptors present human fibroblasts, monocyte-derived macrophages and rat peritoneal macrophages. isolated LDL ( d = 1.019−1.063 g / ml ) carbamylated different numbers lysine residues tested cell-interactive properties, including binding, degradation, stimulation [ 3 H]oleate incorporation into cholesteryl oleate. Small carbamylation (approximately 1–2% residues) resulted in a reduced ability (70–80% control) to displace 125 I-labeled from fibroblast receptors. Modification 12.5–25% marked increase interact scavenger an almost total loss apolipoprotein B-E Acetylated malondialdehyde-modified inhibited competitively degradation I-carbamylated by Thus, extent plays important role recognition modified There also seems be range over which is not yet recognized receptor, but receptor markedly reduced. This perhaps explains how small vivo can result residence time subendothelial tissue lead further local interactions, ultimately increasing atherogenicity particle.