Determination of 25-hydroxyvitamin D in human plasma using high-performance liquid chromatography--tandem mass spectrometry.

摘要: We report here the development of a precise and sensitive method to determine 25-hydroxyvitamin D (25-OH-D2/ -D3) in human plasma using high-perfomance liquid chromatography−tandem mass−mass spectrometry with atomospheric pressure chemical ionization (LC-APCI-MS/MS). The involves use deuterated 25-OH-D3 as an internal standard compound for 25-OH-D2/-D3, which was synthesized our laboratory, selection precursor product ion MS/MS multiple reaction monitoring method. average intraassay interassay variation values (relative deviation) were 5.7 2.5%, respectively, 4.5 5.1%, 25-OH-D2. spiked recoveries from authentic compounds added normal samples 25-OH-D2 103.8 98.8%, respectively. Mean concentrations healthy subjects 20.5 0.4 ng/mL, conclude that this novel LC-APCI-MS/MS would be useful for...