Basic protein and peptide protocols

摘要: The Lowry Method for Protein Quantitation. Bicinchoninic Acid (BCA) Assay Bradford Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins. SDS Gradient Electrophoresis. Acetic Acid-Urea Peptides. Isoelectric Focusing Proteins in Ultra-Thin Gels. In Vivo Isotopic Labeling Two-Dimensional Using Immobilized pH Gradients the First Dimension. Flat-Bed Quantification on Gels (Nonradioactive). Detection an Ultrasensitive Silver Staining Technique. Identification Glycoproteins Nitrocellulose Membranes and Release Oligosaccharides from Glycoproteins. Structural Profiling Drying Fluorography Containing Tritium. Electrophoretic Elution Preparation Microsequencing. Comparison Primary Structures: Peptide Mapping. Blotting. Polypeptides Immunoblots Secondary Antibodies or A. Blots Avidin-Biotin System. Chemiluminescent Systems. Gold Immunogold. Amino Analysis. Molecular-Weight Estimation Native Size-Exclusion High-Pressure Liquid Chromatography. Purification by Reverse-Phase HPLC. Enzymatic Methods Cleaving Chemical Cleavage Modification Dansyl Identifying N-Terminal Acids. Dansyl-Edman Sequencing. C-Terminal Sequencing Peptides: Thiocyanate Degradation Method. Analysis Cysteine Residues Disulfide Bonds. Characterization Monoclonal to Other Cellular Components. Raising a Polyclonal Antiserum Protein. Production Antisera Synthetic Purified Immunoglobulin G (IgG). Enzyme Biotin Antibody. Peptides Radioiodination. Radioimmunoassay Enzyme-Linked Immunosorbant (ELISA). Free Zone Capillary Index.