Integrated analysis of transcriptome and metabolites reveals an essential role of metabolic flux in starch accumulation under nitrogen starvation in duckweed

摘要: Duckweed is considered a promising source of energy due to its high starch content and rapid growth rate. Starch accumulation in duckweed involves complex processes that depend on the balanced expression genes controlled by various environmental endogenous factors. Previous studies showed nitrogen starvation induces global stress response results duckweed. However, relatively little known about mechanisms underlying regulation under conditions starvation. In this study, we used next-generation sequencing technology examine transcriptome responses Lemna aequinoctialis 6000 at three stages (0, 3, 7 days) during presence exogenously applied sucrose. Overall, 2522, 628, 1832 differentially expressed unigenes (DEGs) were discovered for treated control samples. Clustering enrichment analysis DEGs revealed several biological occurring Genes involved metabolism earliest starvation, whereas carbohydrate biosynthesis responded subsequently. The encoding nitrate reductase, glutamine synthetase, glutamate synthase was down-regulated enzymes gluconeogenesis up-regulated, while majority glycolysis down-regulated. metabolite more ADP-Glc accumulated lower levels UDP-Glc activity AGPase significantly increased UGPase dramatically decreased. These changes are roughly consistent with gene transcriptome. Based these results, it can be concluded increase ADP-glucose contents consequence output from TCA pathways, accompanied reduction lipids pectin biosynthesis. provide novel insights into which foundation improvement advanced bioethanol production