Magnetic nanoparticles as a new approach to improve the efficacy of gene therapy against differentiated human uterine fibroid cells and tumor-initiating stem cells.

摘要: Objective To study whether efficient transduction and subsequent elimination of fibroid tumor-initiating stem cells during debulking tumor will aid in completely eradicating the as well decreasing likelihood recurrence. Design Case control study. Setting Research laboratory. Patient(s) None. Intervention(s) Magnetic nanoparticles (MNPs) complexed to adenovirus (Ad-GFP) or (Ad-LacZ) used transfect differentiated human in vitro. Main Outcome Measure(s) Rate growth inhibition. Result(s) We have developed a localized nonsurgical adenovirus-based alternative for treatment uterine fibroids that combines viral-based gene delivery with nanotechnology more targeting. adenovirus, presence an external magnetic field, accelerate transduction. observed statistically significant increase efficiency among at two different multiplicities infection (MOI), 1 10, respectively, MNPs compared alone. Human transfected Ad-LacZ expressed β-galactosidaze MOI 1, 50 19%, 62%, 90%, which were significantly enhanced MNPs. Conclusion(s) When applied herpes simplex thymidine kinase, magnetofection suppressed proliferation induced apoptosis both cell types. Through use magnetofection, we prove lower viral dose effectively overall safety profile suicide therapy against tumors.