Gene expression profiling of the pre-implantation mouse embryo by microarray analysis: comparison of the two-cell stage and two-cell block.

摘要: To improve our understanding of the molecular mechanisms underlying early embryo development, further characterization gene activity in oocytes and embryos is urgently required. The transition from two-cell to four-cell stage particularly important pre-implantation embryonic as it involves transcriptional reprogramming cellular differentiation. In this study, we used a 7.4 K cDNA microarray screen mRNA transcript levels mouse embryo. Real-time PCR was confirm data. We profiled 7410 genes identified 4562 that were differentially expressed selected total 248 with significant expression changes are functionally involved block Of these genes, 114 down-regulated remainder (n = 134) up-regulated This study provides developmental map large number particular emphasis on Further investigations based data will provide better effects various external conditions may facilitate comparative analysis development other mammalian species, including human.