Isolation of Escherichia coli O157:H7 from retail fresh meats and poultry.

摘要: A total of 896 samples retail fresh meats and poultry was assayed for Escherichia coli serogroup O157:H7 by a hydrophobic grid membrane filter-immunoblot procedure developed specifically to isolate the organism from foods. The involves several steps, including selective enrichment, filtration enrichment culture through filters, incubation each filter on nitrocellulose paper agar, preparation an immunoblot (by using antiserum E. filtrate) paper, selection filters colonies which corresponded immunopositive sites blots, screening isolates Biken test precipitin lines metabolites filtrate, confirmation as Vero cell cytotoxic biochemical, serological, cytotoxicity tests. isolated 6 (3.7%) 164 beef, 4 (1.5%) 264 pork, 263 poultry, (2.0%) 205 lamb samples. One 14 pork 5 17 beef contaminated with were Calgary, Alberta, Canada, grocery stores, whereas all other Madison, Wis., outlets. This is first report isolation food than ground results indicate that not rare contaminant poultry.