Screening methods in eucaryotic cells
作者： Allan Frankel , Ruoying Tan
DOI:
关键词:
摘要: The invention provides new methods for screening libraries of peptides and other compounds a desired property in eucaryotic cells. are premised, part, on the unexpected observation that contents procaryotic or lower cells, such as yeast, can be transferred to recipient cells an essentially clonal manner by protoplast fusion respective Applications include substantially incapable episomal replication nucleic acid fragments; secondary metabolites produced cells; library capacity bind selected RNA
lens.org 参考文章(22)
Minoo Rassoulzadegan, Bernard Binetruy, François Cuzin, High frequency of gene transfer after fusion between bacteria and eukaryotic cells. Nature. ,vol. 295, pp. 257- 259 ,(1982) , 10.1038/295257A0
Kazuo Harada, Shelley S. Martin, Alan D. Frankel, Selection of RNA-binding peptides in vivo Nature. ,vol. 380, pp. 175- 179 ,(1996) , 10.1038/380175A0
W. Schaffner, Direct transfer of cloned genes from bacteria to mammalian cells Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 2163- 2167 ,(1980) , 10.1073/PNAS.77.4.2163
John L. Yates, Noreen Warren, Bill Sugden, Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells Nature. ,vol. 313, pp. 812- 815 ,(1985) , 10.1038/313812A0
James B. Allen, Mark W. Walberg, Michael C. Edwards, Stephen J. Elledge, Finding prospective partners in the library: the two-hybrid system and phage display find a match Trends in Biochemical Sciences. ,vol. 20, pp. 511- 516 ,(1995) , 10.1016/S0968-0004(00)89119-7
J. Li, I Herskowitz, Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system. Science. ,vol. 262, pp. 1870- 1874 ,(1993) , 10.1126/SCIENCE.8266075
T Lufkin, C Bancroft, Identification by cell fusion of gene sequences that interact with positive trans-acting factors. Science. ,vol. 237, pp. 283- 286 ,(1987) , 10.1126/SCIENCE.3474782
Chaitanya Jain, Joel G Belasco, A structural model for the HIV-1 Rev-RRE complex deduced from altered-specificity rev variants isolated by a rapid genetic strategy. Cell. ,vol. 87, pp. 115- 125 ,(1996) , 10.1016/S0092-8674(00)81328-8
James E. Wilhelm, Ronald D. Vale, A one-hybrid system for detecting RNA-protein interactions. Genes to Cells. ,vol. 1, pp. 317- 323 ,(1996) , 10.1046/J.1365-2443.1996.25026.X
Brent E. Korba, John F. Lechner, George H. Yoakum, Curtis C. Harris, Protoplast fusion method for high-frequency DNA transfection in human cells ,(1983)