Numb negatively regulates the epithelial-to-mesenchymal transition in colorectal cancer through the Wnt signaling pathway

摘要: Colorectal cancer (CRC) is one of the most common malignant tumors and associated with a high mortality rate due to lack specific biomarkers available for early diagnosis, targeted therapies, prognostic surveillance. In present study, we investigated function Numb its underlying mechanism in CRC. Immunohistochemical staining clinicopathological analysis were used assess expression clinical significance patients Quantitative real-time polymerase chain reaction, cell proliferation, Western blot, wound healing, Transwell, TOP/FOP flash reporter assays investigate was downregulated negatively correlated depth invasion, tumor size, metastasis, TNM stage, epithelial-to-mesenchymal transition (EMT) markers CRC specimens. regulates EMT, migration, Wnt signaling pathway vitro, as well growth metastasis vivo. Furthermore, activation by Wnt-3A negated effect overexpression, whereas inhibition IWR-1 impaired knockdown on EMT. We concluded that downregulation event closely progression poor prognosis. functions suppressor CRC, mediated negative regulation EMT through pathway.NEW & NOTEWORTHY colorectal quantitative assays. conclude can regulate inhibit development cancer.