Characterization of Some Cylindrocladium Species with Three-Septate Conidia Using Morphology, Isozyme Banding Patterns and DNA Polymorphisms

作者: P.W. Crous , B.J.H. Janse , D. Victor , G.F. Marais , A.C. Alfenas

DOI: 10.1016/S0723-2020(11)80479-0

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摘要: Summary Morphological and growth characteristics, esterase isozyme banding patterns total DNA profiles were obtained for isolates of Cylindrocladium citri Fawcett & Klotz, C. crotalariae (Loos) Bell Sobers, ilicicola (Hawley) Boedijn Reitsma, penicilloides (Tub.) Tubaki, spathulatum El-Gholl, Kimbrough, Barnard, Alfieri Schoulties as well seven previously unidentified isolates. The study zymograms enhanced our ability to determine relationships among within species. Each procedure allowed us differentiate all the species except between citri, which appeared distinct only on basis their zymograms. may therefore be regarded an intraspecific variant citri. Furthermore, a new species, variabile, its teleomorph, Calonectria variabilis, are described. has vesicle morphology, patterns.

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