Electromechanical coupling in the membranes of Shaker-transfected HEK cells

摘要: Membranes flex with changes in transmembrane potential as a result of interfacial tension, the Lippman effect. We studied membrane electromotility Shaker K(+)-transfected HEK-293 cells real time by using combined patch-clamp atomic force microscopy. In voltage range where channels were closed, expression had little effect on relative to wild-type cells. Depolarization between -120 and -40 mV resulted linear upward cantilever deflection equivalent an increase tension. However, when depolarized sufficiently for channel opening, saturated only recovered over 10 s milliseconds. This remarkable loss motility was associated not ionic flux or movement sensors. The IL mutant Shaker, which dependence opening but sensor is shifted more positive potentials, caused saturation also shift potentials. temporary probably local buckling bilayer inner half expands expected from X-ray structural data.