Short history of the “Genomic Revolution” and implication for neurological institutes

摘要: The “original” DNA sequencing methodology is the Sanger method, which has excellent accuracy and reasonable read length but very low throughput. In order to sequence longer sections of DNA, shotgun been introduced for entire human genome in Human Genome Project (HGP). Unfortunately, formidable size diploid prevented routine application methods deciphering complete individual genomes. To realize full potential genomics health, last ten years have high-throughput (or Next-Generation Sequencing, NGS) technologies that parallelizing process, are able improve throughput lower cost sequencing. NGS important, particular if applied neurological disease. This kind disorders complex various ways; multiple risk factors, environmental as well genetic, contribute disorder individually or by means interaction. Also genetic component complex: heterogeneity (production apparently identical different mechanisms genes at loci) described a lot disorders. Therefore analysis this field require time. approaches will allow scan several responsible pathology, faster cheaper than ever, improving quality process year too slow expensive produce molecular information useful clinical management patient.