In Situ Analysis of Cell Populations: Long-Term Label-Retaining Cells

摘要: The mammary gland consists of an epithelial ductal tree embedded in a fat pad. Adult epithelium has been demonstrated to have outstanding regenerative potential, consistent with the presence resident, adult stem cells. However, there are currently no bona fide markers identify these cells within their tissue context. Here, we introduce long-term label retention as method investigate location quiescent (a property attributed cells) situ. Long-term retaining divide actively during development and remain at homeostasis. These two properties Therefore, label-retaining can be used populations that contain We describe materials methods necessary image cells, carry out morphometric analysis on map distribution epithelium. spatial analyses described here generally applicable any cell populations, will therefore useful characterize once become available.