Gene expression profiling of ductal carcinomas in situ and invasive breast tumors.

摘要: Comparative and functional genomics are powerful tools to advance the understanding of molecular basis cancer. It is believed that genes epigenetically regulated and, thus, each tumor type stage will be characterized by a gene expression fingerprint. In this study we identified differentially expressed in ductal carcinoma situ invasive breast. To isolate associated with progression breast cancer performed differential display subtractive cloning procedures using matched RNA from normal tissue. cDNA microarray analysis generated profiles typical transition front when used mRAA extracted case low-to intermediate-grade DCIS high-grade DC1S/IDC. cDNAs these samples were probes hybridization 9183 unique cDAAs representing 8507 genes. Signals both transcriptomes obtained for 8083 genes, balanced values between pure DCIS/invasive tumors revealed 303 distinct ratio > 2. Interferon inducible found at highest level sample. Genes most abundantly immunoglobulin heavy constant gamma 3 calgranulin B. Further protein cell lines patient tissue that: IGFBP-rP1 down-regulated whereas cyclin I ubiquitination ER-negative cancers. Conclusion: The known novel discussed here represent targets characterization during development as well as,for designing strategies diagnosis treatment. (Less)