The class II major histocompatibility complex molecule BoLA-DR is expressed by endothelial cells of the bovine corpus luteum

摘要: Cells expressing class II major histocompatibility complex (MHC) molecules are found within the corpus luteum (CL) of several species. Expression and localization MHC in bovine CL were examined present study. Immunohistochemical evaluation revealed on single cells early (days 4 5 post-estrus). Two MHC-expressing cell types observed midcycle 10-12 post-estrus), similar to those CL, endothelial cells. Not all expressed MHC, further investigation expression only one type molecule, DR, Class was also localized late (day 18 Steroidogenic luteal negative for throughout estrous cycle. Quantitative RT-PCR higher (P < 0.05) concentrations mRNA encoding alpha-subunit DR (DRA) when compared with CL. DRA abundance measured cultures mixed (CLENDO) cells, presence or absence tumor necrosis factor-alpha (TNF). No differences concentration between CLENDO cultures, TNF had no effect both types. by may induce anergy T lymphocytes, stimulate them secrete products that enhance normal function.