Assessment of statistic analysis in non-radioisotopic local lymph node assay (non-RI-LLNA) with α-hexylcinnamic aldehyde as an example

摘要: The murine local lymph node assay (LLNA) is used for the identification of chemicals that have potential to cause skin sensitization. However, it requires specific facility and handling procedures accommodate a radioisotopic (RI) endpoint. We developed non-radioisotopic (non-RI) endpoint LLNA based on BrdU incorporation avoid use RI. Although this alternative method appears viable in principle, somewhat less sensitive than standard assay. In study, we report investigations determine statistical analysis improve sensitivity non-RI procedure with alpha-hexylcinnamic aldehyde (HCA) two separate experiments. Consequently, required HCA concentrations greater 25% elicit positive response criterion classification as sensitizer LLNA. Nevertheless, dose responses were consistent both experiments examined whether an upon significance induced changes LNC turnover, rather SI 3 or greater, might provide additional sensitivity. results reported here demonstrate at least significant were, each experiments, recorded following exposure mice HCA. These data suggest approach may be more satisfactory-at when measured. modification if employing Taken together modified which place radioisotope shows some promise, but its present form, even endpoint, lacks method. challenge develop strategies further refinement approach.