Label-Free Detection of Rare Cell in Human Blood Using Gold Nano Slit Surface Plasmon Resonance

摘要: Label-free detection of rare cells in biological samples is an important and highly demanded task for clinical applications various fields research, such as circulating tumor cancer therapy stem studies. Surface Plasmon Resonance (SPR) a label-free method promising technology diagnosis or research applications. Short depth SPR (400 nm) provides sensitive with minimum interference non-targets the samples. In this work, we developed novel microfluidic chip integrated gold nanoslit platform efficient immunomagnetic capturing human blood. Our offers simple yet target high purity. The approach consists two steps. Target are firs captured on functionalized magnetic nanoparticles (MNPs) specific antibody I. suspension containing (MNPs-cells) then introduced into film. MNPs-cells bind second immobilized surface therefore sensor active area. cell binding was monitored by wavelength shift spectrum generated nanoslits.