The cytoplasmic domain of CD4 plays a critical role during the early stages of HIV infection in T-cells.

摘要: Abstract The role played by the cytoplasmic domain of CD4 molecule in process HIV infection was investigated, using A2.01 cells which express different forms gene. A delay production consistently observed expressing a truncated lacks (CD4.401) compared with wild type CD4. The much less hybrid CD4-CD8 (amino acids 1-177 fused to hinge, transmembrane and domains CD8). Yet extent viral entry reverse transcription, monitored semi-quantitative PCR, similar each cell studied. For further study mechanism responsible for delayed replication A2.01/CD4.401 line, were treated phytohaemagglutinin (PHA), 24 h after infection. Under such experimental conditions detected at same time culture supernatants A2.01/CD4 cells. Moreover, we found that oligomerization HIV-1 induced NF-kappa B translocation A2.01/CD4-CD8 but not This consistent CAT assay experiments provided evidence Tat-independent mediated activation LTR promoter binding In contrast results published recently Tremblay et al. (1994, EMBO J., 13, 774-783), propose positive cellular signal initiated following virus itself is involved B-dependent early transcription