Engineering of plant chromosomes

摘要: Engineered minimal chromosomes with sufficient mitotic and meiotic stability have an enormous potential as vectors for stacking multiple genes required complex traits in plant biotechnology. Proof of principle essential steps chromosome engineering such truncation by T-DNA-mediated telomere seeding de novo formation centromeres cenH3 fusion protein tethering has been recently obtained. In order to generate robust protocols application biotechnology, these need be combined supplemented additional methods site-specific recombination the directed transfer interest on minichromosomes. At same time, development allows new insight into basic aspects functions how assure proper distribution daughter cells or telomeres serve cap ends prevent shortening over DNA replication cycles end fusion.