Thermosensitive polymer-based hydrogel mixed with the anti-inflammatory agent minocycline induces axonal regeneration in hemisected spinal cord
作者： Young Mi Kang , Dong Hoon Hwang , Byung Gon Kim , Dong Hyun Go , Ki Dong Park
DOI: 10.1007/S13233-010-0412-5
关键词:
摘要: Bridging lesion cavities with bioengineered scaffolds is a promising strategie for spinal cord repair. In rat model of hemisection, the present study utilized an injectable hydrogel Tetronic-oligolactide (TL) copolymer, which liquid solution at room temperature and gels 37 °C. The implantation TL allowed growth laminin-laden connective tissue matrix formation blood vessels in cavities. However, alone did not significantly increase level axonal through areas. It was hypothesized that macrophage infiltration into areas reduced promoting effect hydrogel. Implanting mixed anti-inflammatory agent, minocycline, decreased extent deposition chondroitin sulfate proteoglycans, can potently inhibit regeneration. Finally, plus minocycline increased length axon dose-dependent manner. These results suggest controlling inflammation improves functionality used as bridging strategy.
springer.com
ajou.ac.kr参考文章(29)
Barbara Grimpe, Jerry Silver, Chapter 23 The extracellular matrix in axon regeneration Progress in Brain Research. ,vol. 137, pp. 333- 349 ,(2002) , 10.1016/S0079-6123(02)37025-0
Byeongmoon Jeong, You Han Bae, Doo Sung Lee, Sung Wan Kim, Biodegradable block copolymers as injectable drug-delivery systems Nature. ,vol. 388, pp. 860- 862 ,(1997) , 10.1038/42218
Leonard L. Jones, Dana Sajed, Mark H. Tuszynski, Axonal Regeneration through Regions of Chondroitin Sulfate Proteoglycan Deposition after Spinal Cord Injury: A Balance of Permissiveness and Inhibition The Journal of Neuroscience. ,vol. 23, pp. 9276- 9288 ,(2003) , 10.1523/JNEUROSCI.23-28-09276.2003
Puckett Wr, Bunge Rp, Quencer Rm, Becerra Jl, Marcillo A, Observations on the pathology of human spinal cord injury : a review and classification of 22 new cases with details from a case of chronic cord compression with extensive focal demyelination Advances in Neurology. ,vol. 59, pp. 75- 89 ,(1993)
Dong Hyun Go, Yoon Ki Joung, Sang Young Lee, Myung Chul Lee, Ki Dong Park, Tetronic-oligolactide-heparin hydrogel as a multi-functional scaffold for tissue regeneration. Macromolecular Bioscience. ,vol. 8, pp. 1152- 1160 ,(2008) , 10.1002/MABI.200800098
David R. Nisbet, Andrew E. Rodda, Malcolm K. Horne, John S. Forsythe, David I. Finkelstein, Neurite infiltration and cellular response to electrospun polycaprolactone scaffolds implanted into the brain Biomaterials. ,vol. 30, pp. 4573- 4580 ,(2009) , 10.1016/J.BIOMATERIALS.2009.05.011
Michael S. Beattie, Inflammation and apoptosis: linked therapeutic targets in spinal cord injury Trends in Molecular Medicine. ,vol. 10, pp. 580- 583 ,(2004) , 10.1016/J.MOLMED.2004.10.006
Sergey G. Kremlev, Rebecca L. Roberts, Charles Palmer, Minocycline modulates chemokine receptors but not interleukin-10 mRNA expression in hypoxic-ischemic neonatal rat brain. Journal of Neuroscience Research. ,vol. 85, pp. 2450- 2459 ,(2007) , 10.1002/JNR.21380
Karin S. Straley, Cheryl Wong Po Foo, Sarah C. Heilshorn, Biomaterial Design Strategies for the Treatment of Spinal Cord Injuries Journal of Neurotrauma. ,vol. 27, pp. 1- 19 ,(2010) , 10.1089/NEU.2009.0948
Michael T. Fitch, Jerry Silver, Activated macrophages and the blood-brain barrier: inflammation after CNS injury leads to increases in putative inhibitory molecules Experimental Neurology. ,vol. 148, pp. 587- 603 ,(1997) , 10.1006/EXNR.1997.6701