Polymorphism of prolactin, growth differentiation factor 9, and calpastatin genes and their effects on weight traits in Awassi lambs.

作者: Khaleel Jawasreh , Zuhair Ismail

DOI: 10.5455/JAVAR.2019.F317

关键词:

摘要: Objectives This study was conducted to determine the correlation among prolactin gene (PRG), growth differentiation factor 9 (GDF-9), and calpastatin (CAG) genes polymorphism with traits in Awassi lambs. Materials methods Records of 779 lambs from 264 dams 15 sires were used. The sex, type birth (single versus twins), weight at birth, average daily gain (ADG), adjusted 60 days age determined for each animal. Blood samples collected all PRG, GDF-9, CAG using polymerase chain reaction-Restriction fragment length polymorphism. Results Three PRG genotypes (AA, AB, BB) a frequency 0.88, 0.05, 0.07, respectively. allele 0.84 0.16 A B alleles, For there only two detected (AB 0.96 0.04, 0.92 0.08 CAG, also 0.08, 0.04 significant (p ≤ 0.04) effect genotype on but this not ADG weaning. There no associations between any pre-weaning GDF-9 variants. Conclusion results show that could be used select high dystocia reduce newborn therefore conserve dam's reproductive functions improve lamb survivability.

参考文章(13)
E. Liandris, A. Kominakis, M. Andreadou, K. Kapeoldassi, S. Chadio, Th. Tsiligianni, M. Gazouli, I. Ikonomopoulos, Associations between single nucleotide polymorphisms of GDF9 and BMP15 genes and litter size in two dairy sheep breeds of Greece Small Ruminant Research. ,vol. 107, pp. 16- 21 ,(2012) , 10.1016/J.SMALLRUMRES.2012.04.004
M. Orford, O. Tzamaloukas, C. Papachristoforou, D. Miltiadou, Technical note: A simplified PCR-based assay for the characterization of two prolactin variants that affect milk traits in sheep breeds. Journal of Dairy Science. ,vol. 93, pp. 5996- 5999 ,(2010) , 10.3168/JDS.2010-3569
E.A. Staiger, M.L. Thonney, J.W. Buchanan, E.R. Rogers, P.A. Oltenacu, R.G. Mateescu, Effect of prolactin, β-lactoglobulin, and κ-casein genotype on milk yield in East Friesian sheep Journal of Dairy Science. ,vol. 93, pp. 1736- 1742 ,(2010) , 10.3168/JDS.2009-2630
K. I. Z. Jawasreh, H. Ababneh, F. T. Awawdeh, M. A. Al-Massad, A. M. Al-Majali, Genotype and allelic frequencies of a newly identified mutation causing blindness in jordanian awassi sheep flocks. Asian-australasian Journal of Animal Sciences. ,vol. 25, pp. 33- 36 ,(2011) , 10.5713/AJAS.2011.11187
James P. Hanrahan, Scott M. Gregan, Philippe Mulsant, Michael Mullen, George H. Davis, Richard Powell, Susan M. Galloway, Mutations in the Genes for Oocyte-Derived Growth Factors GDF9 and BMP15 Are Associated with Both Increased Ovulation Rate and Sterility in Cambridge and Belclare Sheep (Ovis aries) Biology of Reproduction. ,vol. 70, pp. 900- 909 ,(2004) , 10.1095/BIOLREPROD.103.023093
sutikno sutikno, M Yamin, C Sumantri, Association of Polymorphisms Calpastatin Gene with Body Weight of Local Sheep in Jonggol, Indonesia Media Peternakan. ,vol. 34, pp. 1- 6 ,(2011) , 10.5398/MEDPET.2011.34.1.1
M Mamoee, AS Sadr, M Mohammadi, K Alami-Saeid, Mtb Nasiri, J Fayazi, Polymorphism of calpastatin gene in Arabic sheep using PCR- RFLP African Journal of Biotechnology. ,vol. 7, ,(2008) , 10.4314/AJB.V7I15.59121
A.M. Ramos, C.A.P. Matos, P.A. Russo-Almeida, C.M.V. Bettencourt, J. Matos, A. Martins, C. Pinheiro, T. Rangel-Figueiredo, Candidate genes for milk production traits in Portuguese dairy sheep Small Ruminant Research. ,vol. 82, pp. 117- 121 ,(2009) , 10.1016/J.SMALLRUMRES.2009.02.007
R. J. Kerr, B. P. Kinghorn, An efficient algorithm for segregation analysis in large populations Journal of Animal Breeding and Genetics. ,vol. 113, pp. 457- 469 ,(1996) , 10.1111/J.1439-0388.1996.TB00636.X