Effect of tumor necrosis factor α and β on human oligodendrocytes and neurons in culture

摘要: Abstract Cytokines produced by infiltrating hematogenous cells or glial activated during the course of central nervous system disease trauma are implicated as mediators tissue injury. In this study, we have assessed extent and mechanism injury human-derived CNS oligodendrocytes neurons in vitro mediated cytokines tumor necrosis factor α β compared these with independent effects CD4 + T-cells. We found that T-cells, but not β, could induce significant release lactate dehydrogenase, a measure cell membrane lysis, from within 24 hr. Neither induced DNA fragmentation measured using fluorescence nick-end labelling technique. After more prolonged time period (96 hr), did nuclear changes proportion without increased dehydrogenase release. The was comparable to serum deprivation. Tumor were even pronounced. contrast oligodendrocytes, fragmentation, propidium iodide staining, less than In-situ hybridization studies human adult culture indicated astrocytes, well microglia, can express mRNA.