Both linopirdine- and WAY123,398-sensitive components of I K(M,ng) are modulated by cyclic ADP ribose in NG108-15 cells.

摘要: The "M-like" current in NG108–15 cells has two components carried by different K+ channels: a fast-deactivating component, analogous to I K(M) sympathetic neurones and KCNQ2/3 channels, more slowly deactivating component murine erg1 (merg1) channels. former is selectively blocked linopirdine (≤10 µM), the latter WAY123,398 (≤10 µM). Bradykinin (100 nM) inhibited 76% of KCNQ compared with 12% merg component. Cyclic ADP ribose (cADPR, 2 µM), introduced via patch pipette, caused rundown both components. Acetylcholine (100 µM) 89% 34% After 15 min intracellular dialysis cADPR antagonist 8-amino-cADP (100 µM), inhibition reduced 40% 19% after 30 min it was further 8% 5% for currents respectively. These data show that can be modulated either bradykinin or M1 muscarinic receptors. pronounced than results suggest might involved M1-muscarinic merg1