Analysis of p60 and p80 tumor necrosis factor-alpha receptor messenger RNA and protein in human placentas.

摘要: Tumor necrosis factor-alpha (TNF), a pleiotrophic, multifunctional polypeptide factor, has been reported in both normal and infected human placentas. To identify potential targets for this cytokine, the cells early late gestation placentas extraplacental membranes that express two TNF receptor (TNF-R) genes, p60 p80, were identified by using situ hybridization immunocytochemistry. Gestation-related, cell lineage-specific differences steady-state levels of p80 TNF-R messenger RNA observed. predominated at stages gestation, being high mesenchymal trophoblastic lineages. By contrast, was abundant only intermittent stretches first trimester syncytiotrophoblast term placental cells. Overall, intensities signals stronger than tissues. Transcription genes confirmed Northern blot hybridization. Translation verified all samples immunohistology polyclonal antibodies specific proteins. proteins intracellularly maternal fetal blood. Because TNF-Rs exist membrane-bound soluble forms, results study are consistent with postulate have critical functions: 1) modulation utilization lineages during course pregnancy; 2) protection against excessive produced infections.