Intranasal administration of plasmid DNA-coated nanoparticles results in enhanced immune responses

摘要: Intranasal immunization offers potential forthe elicitation of effective mucosal and systemic immune responses. In this study, a previously reported novel cationic nanoparticle engineered from microemulsion precursor was further modified, optimized applied intranasally to mice explore its as plasmid DNA (pDNA) vaccine delivery system. To end, more uniform nanoparticles (around 100 nm) containing less surfactant were developed. The pDNA-coated significantly enhanced the specific serum IgG IgA titres an expressed model antigen, beta-galactosidase, by 18-28 25-30 fold, respectively, when compared with naked pDNA alone. An splenocyte proliferative response also observed after nanoparticles. It concluded that these DNA-coated may have for via nasal route.