Isolation and characterization of a gamma-type phosphoinositide-specific phospholipase C (PLC-gamma 2).
作者： Y Homma , Y Emori , F Shibasaki , K Suzuki , T Takenawa
DOI: 10.1042/BJ2690013
关键词:
摘要: A novel bovine spleen phosphoinositide-specific phospholipase C (PLC) has been identified with respect to immunoreactivity four independent antibodies against each of the PLC isoenzymes, and purified near homogeneity by sequential column chromatography. Spleen contains three isoenzymes: two different gamma-types [gamma 1 gamma 2, originally named as PLC-gamma [Rhee, Suh, Ryu & Lee (1989) Science 244, 546-550] PLC-IV [Emori, Homma, Sorimachi, Kawasaki, Nakanishi, Suzuki Takenawa J. Biol. Chem. 264, 21885-21890] respectively] delta-type enzyme, but is separated from 2 pool first DEAE-cellulose Subsequently, PLC-delta dissociated on third heparin-Sepharose The enzyme a molecular mass 145 kDa SDS/polyacrylamide-gel electrophoresis specific activity 12.8 mumol/min per mg phosphatidylinositol 4,5-bisphosphate substrate. This dependent Ca2+ for hydrolysis all these phosphoinositides. None other phospholipids examined could be its substrate at any concentration Ca2+. optimal pH slightly acidic (pH 5.0-6.5).
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