Photodynamic antibacterial enhanced effect of methylene blue-gold nanoparticles conjugate on Staphylococcal aureus isolated from impetigo lesions in vitro study

摘要: Background Staphylococcal aureus is the most common organism which has been encountered in impetigo infection. Gold nanoparticles can be used as a tool to deliver antimicrobials or enhance photodynamic destruction of bacteria. Objective To evaluate effect methylene blue gold (MB-gold conjugate) on S. were isolated from lesions. Patient and methods Twenty children diagnosed clinically impetigo, aged 3 5 years both sexes recruited study. Two bacteriological samples collected each patient, identified cultured. Samples concentration 10−1 ml divided into four groups. was treated by MB-gold conjugate, nanoparticles, MB, fourth group served control group. Diode laser (660 nm) for photoactivation. The bacterial growth inhibition determined two methods: percentage reduction viable bacteria count optical density (O.D) growth. Results The highest significant inhibitory obtained with conjugate when irradiated diode 660 nm (P < 0.0001). 3%. photoactivated showed (P < 0.05). A non-significant elicited other groups. Conclusion The maximum activity. proved efficacy drug delivery system. It enhanced antibacterial blue.