Heat treatment alleviates the growth and photosynthetic impairment of transplastomic plants expressing Leishmania infantum Hsp83-Toxoplasma gondii SAG1 fusion protein

摘要: Previously, we showed that transplastomic tobacco plants expressing the LiHsp83-SAG1 fusion protein displayed a chlorotic phenotype and growth retardation, while SAG1 GRA4 antigens alone did not. We conducted comprehensive examination of metabolic photosynthetic parameters could be affecting normal in order to understand origin these pleiotropic effects. These presented all pigments related PSII efficiency significantly diminished. However, expression CHLI, RSSU LHCa/b genes not show significant differences between control plants. Total protein, starch, soluble sugar contents were also greatly reduced Since Hsp90 s are constitutively expressed at much higher concentrations high temperatures, tested if fitness over-expressing LiHsp83 would improve after heat treatment. an important alleviation their evident recovery function. As far as know, this is first report where it demonstrated line performs better temperatures. Finally, detected binding key photosynthesis-related proteins 37 °C. Our results suggest excess molecular chaperone benefit plant possible shock prevent expected denaturation proteins. content was weakly decreased heat-treated Therefore, cannot rule out observed 37 °C may partially due reduction levels recombinant protein.