An applied synchronous fluorescence spectrophotometric assay to study benzo[a]pyrene-diolepoxide-DNA adducts

摘要: Synchronous scanning fluorescence with a fixed wavelength difference (delta lambda) of 34 nm between excitation and emission was used to quantitate benzo[a]pyrene-diol epoxide (BPDE)-DNA adducts. Fluorescence maxima occurred at 382 for BPDE-DNA 379 benzo[a]pyrene-tetrols -triol, which are hydrolysis products BPDE. Similarly, the peak pyrene 372 1-nitropyrene 386 nm. The minimum detectable amount BPDE-moieties in vitro modified BPDE-DNA, after HCl, 20 fmol 100 micrograms DNA, is equivalent 1 adduct per 1.4 X 10(7) nucleotides. correlation intensity linear pmol. DNA isolated from human lymphoblasts incubated BPDE had same incubation mixture linear. Among DNA-samples peripheral blood lymphocytes 30 aluminum plant workers, only one sample found contain similar BPDE-DNA. None 10 persons not occupationally exposed were positive. Measurement adducts by synchronous spectrophotometry should be useful monitoring exposure benzo[a]pyrene.