Improvement of Brazilian bioethanol production - Challenges and perspectives on the identification and genetic modification of new strains of Saccharomyces cerevisiae yeasts isolated during ethanol process.

摘要: In Brazil, bioethanol is produced by sucrose fermentation from sugarcane Saccharomyces cerevisiae in a fed-batch process that uses high density of yeast cells (15-25 % wet weight/v) and sugar concentration (18-22 total sugars). Several research efforts have been employed to improve the efficiency this through isolation yeasts better adapted Brazilian conditions. Two important wild strains named CAT-1 PE-2 were isolated during responsible for almost 60 ethanol production Brazil. However, last decade fermentative substrate composition was much modified, since new cane crops developed, use molasses instead juice increase with prohibition burning prior harvest. As consequence, these previously are being replaced most plants. scenario novel improved characteristics still big challenge. Here, we discuss main aspects selection, characterization genetic modifications phenotypic traits such as thermotolerance.