The Interferon-Inducible Chemokines MuMig and Crg-2 Exhibit Antiviral Activity In Vivo

摘要: MuMig (murine monokine induced by gamma interferon) and Crg-2 (cytokine responsive gene 2) are two murine chemokines of the CXC family that interferons (IFNs): specifically IFN-gamma IFN-alpha, IFN-beta, IFN-gamma. To investigate biological roles these chemokines, recombinant vaccinia viruses (rVVs) encoding either or were constructed. In vitro, chemokine-encoding rVVs replicated to similar levels control virus. Athymic nude mice inoculated with 10(5) PFU less VV-HA-Mig VV-HA-Crg-2 resolved infection successfully whereas given a dose virus VV-HA-TK died from generalized infection. At higher doses, there was mortality in all groups but death significantly delayed infected rVV compared those Virus-encoded enhanced cytolytic activity NK cells splenic cellularity two- threefold resulted significant increases mononuclear cell infiltration livers mice. Using specific neutralizing depleting antibodies, we have established replication athymic mice, as consequence virus-expressed Crg-2, requires