Sex-steroid receptors, prostaglandin E2 receptors, and cyclooxygenase in the equine cervix during estrus, diestrus and pregnancy: Gene expression and cellular localization

摘要: The cervix is a dynamic structure that undergoes dramatic changes during the estrous cycle, pregnancy and parturition. It well established hormonal changes, including estrogens, progestogens prostaglandins, regulate expression of key proteins involved in cervical function. arachidonic acid cascade important remodeling relaxation days preceding Despite complexity this mechanism, regulation function has received little study mare. Therefore, objective was to compare estrogen receptor α (ESR1) β (ESR2), progesterone (PGR), prostaglandin E2 type 2 (PTGER2) 4 (PTGER4) receptors as cyclooxygenase-1 (PTGS1) -2 (PTGS2) equine mucosa stroma estrus, diestrus late using qPCR. Immunohistochemistry used localize ESR1, ESR2, PGR, PTGER2 PTGER4 these regions cervix. Relative mRNA ESR1 PGR greater estrus than both Expression highest compared either or diestrus. Moreover, PTGS1 PTGS2 with but not Immunostaining for consistently detected nucleus cytoplasm epithelium endocervix smooth muscle all stages evaluated. Immunolabeling nuclei pregnancy, ESR2 stages. noted gestation likely reflect preparation subsequent