The pattern of cervical penetration and the effect of topical treatment with prostaglandin and/or FSH and oxytocin on the depth of cervical penetration in the ewe during the peri-ovulatory period.

摘要: Artificial insemination in sheep has two major limiting factors: the poor quality of frozen-thawed ram semen and convoluted anatomy cervix that does not allow transcervical passage an inseminating catheter. It been demonstrated ewe during estrus, there is a degree cervical relaxation mediated by ovarian possibly gonadotrohic hormones, we set out to investigate factors might enhance relaxation. Five experiments were conducted on ewes different breeds determine: 1) pattern penetration periovulatory period several (Welsh Mountain, Ile-de-France, Vendeenne, Romanov Sarda); 2) effect “ram effect” socio-sexual stimulus, penetration; 3) effects intracervical administration follicle-stimulating hormone (FSH), oxytocin prostaglandin E agonist (misoprostol) depth period. The results showed all examined, was increased canal (P < 0.05) Cervical maximum 54 h after removal progestagen sponges then gradually declined. Furthermore, but its pattern, affected breed ewe. lower Vendeenne compared Ile-de-France breeds, which did differ from one another. In presence rams, at 48 reduced 72 0.05). local hormones FSH, misoprostol (a PGE agonist) alone various combinations have any significant conclusion, natural observed occurs time sponges, most suitable for artificial insemination. enhanced even though PGE2 are involved function. preovulatory (54 h) coincides with high LH estradiol concentrations suggesting they be responsible probably through oxytocin-PGE pathway.