Methylation analysis of polysaccharides: Technical advice
作者： Ian M. Sims , Susan M. Carnachan , Tracey J. Bell , Simon F.R. Hinkley
DOI: 10.1016/J.CARBPOL.2017.12.075
关键词:
摘要: Glycosyl linkage (methylation) analysis is used widely for the structural determination of oligo- and poly-saccharides. The procedure involves derivatisation individual component sugars a polysaccharide to partially methylated alditol acetates which are analysed quantified by gas chromatography-mass spectrometry. positions each sugar can be determined correctly identifying acetates. Although methods well established, there many technical aspects this both careful attention detail considerable experience required achieve successful methylation interpret data generated. aim article provide details critical procedural steps necessary assist researchers (a) with interpreting (b) in providing comprehensive reviewers fully assess work.
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