Validation of Tandem Mass Spectrometry Database Search Results Using DTASelect

作者: Daniel Cociorva , David L. Tabb , John R. Yates

DOI: 10.1002/0471250953.BI1304S16

关键词:

摘要: DTASelect provides a means by which complex SEQUEST results can be filtered, organized, and viewed. A single sample may produce tens of thousands tandem mass spectra. Manually perusing selecting matches among such data carries risk inconsistency. allows the user to set criteria for acceptance or rejection individual spectrum results. It also features rules dealing with multiple, identical peptide removing proteins that are insufficiently evidenced. its sorted filtered summary as HTML text documents easy review offers several auxiliary reports. is powerful tool automatic analysis mixture spectrometry.

参考文章(7)
Michael P. Washburn, Dirk Wolters, John R. Yates, Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nature Biotechnology. ,vol. 19, pp. 242- 247 ,(2001) , 10.1038/85686
David L. Tabb, W. Hayes McDonald, John R. Yates, DTASelect and Contrast: Tools for Assembling and Comparing Protein Identifications from Shotgun Proteomics Journal of Proteome Research. ,vol. 1, pp. 21- 26 ,(2002) , 10.1021/PR015504Q
Alexey I. Nesvizhskii, Andrew Keller, Eugene Kolker, Ruedi Aebersold, A statistical model for identifying proteins by tandem mass spectrometry. Analytical Chemistry. ,vol. 75, pp. 4646- 4658 ,(2003) , 10.1021/AC0341261
Jimmy K. Eng, Ashley L. McCormack, John R. Yates, An Approach to Correlate Tandem Mass Spectral Data of Peptides with Amino Acid Sequences in a Protein Database Journal of the American Society for Mass Spectrometry. ,vol. 5, pp. 976- 989 ,(1994) , 10.1016/1044-0305(94)80016-2
Andrew Keller, Alexey I. Nesvizhskii, Eugene Kolker, Ruedi Aebersold, Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search. Analytical Chemistry. ,vol. 74, pp. 5383- 5392 ,(2002) , 10.1021/AC025747H
W. Hayes McDonald, David L. Tabb, Rovshan G. Sadygov, Michael J. MacCoss, John Venable, Johannes Graumann, Jeff R. Johnson, Daniel Cociorva, John R. Yates, MS1, MS2, and SQT—three unified, compact, and easily parsed file formats for the storage of shotgun proteomic spectra and identifications Rapid Communications in Mass Spectrometry. ,vol. 18, pp. 2162- 2168 ,(2004) , 10.1002/RCM.1603
Junmin Peng, Joshua E. Elias, Carson C. Thoreen, Larry J. Licklider, Steven P. Gygi, Evaluation of multidimensional chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) for large-scale protein analysis: the yeast proteome. Journal of Proteome Research. ,vol. 2, pp. 43- 50 ,(2003) , 10.1021/PR025556V