Non-competitive Immunoassays for Small Molecules—the Anti-complex and Selective Antibody Systems

摘要: This chapter explains how high performance immunometric assays can be designed for low molecular weight analytes. It highlights the fundamental limitations of competitive immunoassays and why it is better to measure bound antigen rather than unbound antigen. The anti-complex system most similar conventional assays, with a detection antibody raised against combined antigen–capture complex. selective more complex approach. principle that primary small analyte secondary this are selected such still bind when has molecule but not when, in absence analyte, specific blocking substance. However, an present antibody, binding between antibodies takes place. universal involves generic anti-immunoglobulin as speeding up development new Examples products use these systems provided data.