Anti-metatype Antibody Stabilization of Fv 4-4-20 Variable Domain Dynamics

摘要: Anti-metatype (anti-Met) antibodies are immunoglobulins that specifically recognize and stabilize in their liganded or metatypic state, but lack specificity for either the hapten unliganded antibody. Autologous anti-Met were previously observed vivo, suggesting a autoantibody response could play physiological role immune network, e.g. controlling clearance of complexes from circulation. The first elicited against fluorescein-liganded high affinity murine anti-fluorescein monoclonal antibody 4-4-20. fluorescein-hapten system has proved to be an invaluable tool both recognition characterization by utilization its spectral properties. In this investigation, hydrostatic pressure measurements, conjunction with fluorescence spectroscopy, performed on recombinant Fv derivative (Fv 4-4-20) 4-4-20 complexed study influence intervariable domain interactions. Anti-Met bound cause change properties fluorescein was not when parent immunoglobulin. variation these upon addition shown correlated dissociation variable domains interaction ability dependent whether polyclonal metatype. A model proposed elucidated antibodies, monoclonal, primary anti-antigen