RNA Temperature Demodification

摘要: The extraction of useful RNA from FFPE tissues is often compromised for the efficacy. Moreover, because it resistant to due cross-linking with proteins, in not completely available RT-PCR reactions. RNA, as DNA, indeed modified by presence methylol addition. Prolonged fixations could favor further reactions above-mentioned groups, resulting irreversible artifacts. group doesn’t allow reverse transcription reaction, but majority these groups be removed simply heating extracts formalin-free buffers. This chapter provides two simple temperature treatments demodify obtained FFPE. In our experience demodification treatment seems improve recruitment only old samples. methods described hereafter added routine procedure protocols especially very