Effect of fixatives and tissue processing on the content and integrity of nucleic acids.

作者: Mythily Srinivasan , Daniel Sedmak , Scott Jewell

DOI: 10.1016/S0002-9440(10)64472-0

关键词:

摘要: … and nucleic acids for diagnostic and research purposes. … being directly related to the fixation temperature. Up to 30% of … Acid, 10%: Isolated studies suggest Carnoy's fixative is best …

参考文章(74)
Locker J, Rupp Gm, Purification and analysis of RNA from paraffin-embedded tissues. BioTechniques. ,vol. 6, pp. 56- 60 ,(1988)
J Finke, W Lange, G Dölken, P Ternes, R Fritzen, An improved strategy and a useful housekeeping gene for RNA analysis from formalin-fixed, paraffin-embedded tissues by PCR. BioTechniques. ,vol. 14, pp. 448- 453 ,(1993)
S. Hirohashi, T. Kameya, S. Furuya, K. Mukai, Y. Sato, The AMeX method: a multipurpose tissue-processing and paraffin-embedding method. Extraction of protein and application to immunoblotting. American Journal of Pathology. ,vol. 140, pp. 775- 779 ,(1992)
Koraljka Gall-Trošelj, Maja Herak Bosnar, Mirjana Kardum, Krešimir Pavelić, Jasminka Pavelić, PCR amplification of DNA from archival specimens. A methodological approach. Neoplasma. ,vol. 43, pp. 75- 81 ,(1996)
Alaibac M, Zito Fa, Paradiso A, Schittulli F, Colonna F, Marzullo F, Giannella C, Comparison of formalin, ethanol, and Histochoice fixation on the PCR amplification from paraffin-embedded breast cancer tissue. European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies. ,vol. 35, pp. 633- 635 ,(1997)
John D. Bancroft, Marilyn Gamble, Theory and Practice of Histological Techniques ,(1990)
SHERRY S. ANSHER, RAMI SCHARF, The Cancer Therapy Evaluation Program (CTEP) at the National Cancer Institute: industry collaborations in new agent development. Annals of the New York Academy of Sciences. ,vol. 949, pp. 333- 340 ,(2006) , 10.1111/J.1749-6632.2001.TB04041.X